The Escherichia coli-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice
Figure 2
(a) Expression of recombinant 4 × Tβ4 in BL21 cells at different induction times. Lane M, middle-molecular mass protein markers; lanes from 1 to 5: induced expression of the recombinant 4 × Tβ4 by IPTG (1 mM) at 0, 2, 4, 6, and 8 hs, respectively; lane 6: induced BL21 containing pET28a by IPTG (1 mM) for 6 hs. Red arrows indicate specific band of E. coli-derived 6 × his-×Tβ4. (b) Western blot analysis of the E. coli-derived 6 × his-4 × Tβ4. Lane M: middle-molecular-mass protein markers; lanes from 1 to 5: BL21 harboring pET28a-6 × his-4 × Tβ4 after IPTG (1 mM) induction at 37°C for 0, 2, 4, 6, and 8 h, respectively; lane 6: induction of expression of the BL21 containing pET28a by IPTG (1 mM) at 37°C for 6 h. Red arrows indicate the specific hybridization signal for the E. coli-derived 6 × his-×Tβ4.
