Further Evidence of Mutational Heterogeneity of the XPC Gene in Tunisian Families: A Spectrum of Private and Ethnic Specific Mutations

Table 2

Sequence primers used for PCR amplification of XPC and GAPDH genes.

	PCR	Primer Sense	Primer Anti-sense	𝑇𝑚 °C
XPC	Exon 4	ATGCCTCACTTCCTCCTTCC	CACTTTGATACTCAGTCCTGGTCCC	55
	Exon 5	GATTCACTGTCATCCGAGGAGAAG	CAAAGGCTCAGAGAGAGTAAGAAACTTG	55
	Exon 6	TGAAAGACAAGACCAAAACAAAAACAG	GACCTGAACCCAGCCTCTGAG	55
	Exon 7	CTCCCTCTTTTTATTTTCTTGGCTG	GGTGCCTGTAGGCATTTGATAAAGC	55
	Exon 8	TTGAACAAGCACCATAACAAACAAC	TGCCCAAGTCTTCCCTAACACAG	55
	Exon 9	CCAGGGTGTCTTATAAAGAGG	CAAGGCCTTACCTCCAAG	55
	Exon 10	CCTTGGCTCCACCATCTGTTG	CCCTGTAACTGTTTTTCCCCTGC	60
	Exon 11	AGATTAGGGTTTGTAAGTGGACACATC	GGACTGGGAGGCTCATCATCAC	55
	Exon 12	CTGGTAGGTGTGTTCTGAGGGTTC	CGGTGTAGATTGGGCAGGTTC	60
	Exon 13	GGCAGCATCAGAAGGGCTCAG	AAATCCAGTGTAACATCCTGAAAATTG	60
	Exon 14	AGGCTGGATAGGGGCTTTCAC	CCTGCTGTATTCAGTGCTCGCTC	60
	Exon 15	CCACTAAAGATTTTGGAGTCAGTAACG	ACAGGGCTTGGGGCAGAAGAG	55
	Exon 16	CCCTTGTCCTCCCAGAGTTACAC	ATGCTGCCTCAGTTTGCCTTC	60
	cDNA	TTGAAGAACTTAGTGAGCCTGTG	GCTGGGTTGCCTTCTCCT	60
GAPDH	cDNA	GAGTCAACGGATTTGGTCGT	TTGATT TTGGAGGGATCTCG	60