Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611

Table 2

Extracellular inulinase and invertase productions by different commercial media.

Production media	Extracellular inulinase activity (U/mL)	Extracellular invertase activity (U/mL)
A	157 ± 0.94	536 ± 2.08
B	707 ± 3.14	1167 ± 7.05
C	697 ± 2.16	955 ± 5.02
D	2553 ± 9.17	1983 ± 8.03
E	1664 ± 6.16	1052 ± 6.05
F	880 ± 4.15	663 ± 3.08

A: yeast extract 3 g, malt extract 3 g, peptone 5 g, and dextrose 10 g.
B: potato dextrose broth.
C: for the production of inulinase [inulin (1%), NH₄Cl (2.4%), and Mg₂SO₄·7H₂O (1.2%)] and invertase [inulin (5%), NH₄Cl (4.8%), and Mg₂SO₄·7H₂O (1.2%)].
D: sucrose (20 g), yeast extract (2 g), NaNO₃ (2 g), MgSO₄·7H₂O (0.05 g), and K₂HPO₄ (0.5 g).
E: inulin (10 g), yeast extract (1.5 g), NH₄NO₃ (2.3 g), (NH₄)₂HPO₄ (3.7 g), KH₂PO₄ (1.0 g), and MgSO₄ (0.5 g).
F: inulin (2 g), peptone (2 g), (NH₄)H₂PO₄ (1.2 g), NaCl (0.5 g), and MgSO₄·7H₂O (0.05 g).