Class A 𝜷-Lactamases as Versatile Scaffolds to Create Hybrid Enzymes: Applications from Basic Research to Medicine

Figure 2

Schematic representation of the protocol used to cleave the ChBD domain from BlaP and to purify it. Purified ChBD is collected in the flow through after loading the hybrid protein digested by thrombin on an immobilized metal ion affinity chromatography (IMAC). Figure adapted from Vandevenne et al., 2008 [13]. BlaP-Cter: C-terminal sequence of BlaP, starting at the insertion site. BlaP-Nter: N-terminal sequence of BlaP ending at the insertion site.