p55PIK Transcriptionally Activated by MZF1 Promotes Colorectal Cancer Cell Proliferation

Table 2

Primers for site-directed mutagenesis of construct (−1243/+45) p55PIK.

Name	Sequence change	Sense primer sequence (5′-3′)/antisense primer sequence (5′-3′)
RUNX1-mut	ACCACC to GTGTAT	GCAACCGGCCTCAGCCAATCGTGTATCACCTTGCCTCTCTTCCCTC
		GAGGGAAGAGAGGCAAGGTGATACACGATTGGCTGAGGCCGGTTGC
MZF1-mut1	TGGGGA to CTAGTG	GAAGCCTAGAGAGCGGCTAGTGGACGTGAAAGTCGAG
		CTCGACTTTCACGTCCACTAGCCGCTCTCTAGGCTTC
MZF1-mut2	GGGGG to TAGTC	CGTACCATTCTGATTGAGGCTTAGTCAGAGCGCGGATGGCTGATTGGG
		CCCAATCAGCCATCCGCGCTCTGACTAAGCCTCAATCAGAATGGTACG
MZF1-mut3	TGGGGA to CTAGTG	CGAGAGGCGTGGCTGATCTAGTGGCCGAAGCCTAGAGAGCGG
		CCGCTCTCTAGGCTTCGGCCACTAGATCAGCCACGCCTCTCG
YY1-mut	ACCAT to GTTGA	CCAGCGCCCGGATCGTGTTGATCTGATTGAGGCTGG
		CCAGCCTCAATCAGATCAACACGATCCGGGCGCTGG