Depletion of Luminal Pyridine Nucleotides in the Endoplasmic Reticulum Activates Autophagy with the Involvement of mTOR Pathway
Figure 6
Metyrapone uptake of HepG2 cells. HepG2 cells were treated with 100 μM metyrapone for 0–120 min at 37°C. (a) After cell lysis, cells metyrapone concentrations were measured by HPLC till 120 min upon the addition of metyrapone, and their relative amounts were calculated both in the media (black diamond) and the cells (black triangle). (b) Metyrapone concentration in cells referring to cell volume is plotted at each time point and a trend line was fitted (black hyperbolic line). For detailed description of HPLC method see Section 2.
