Establishment and Evaluation of Stable Cell Lines Inhibiting Foot-and-Mouth Disease Virus by RNA Interference
Figure 4
Decrease of viral yield in transfected cells with different recombinant vectors at the second, fifth, tenth, twentieth, and thirtieth passages. Cells transfected with the shRNAs expressing vectors were infected with FMDV O/CHA/99. Cells-lysed supernatant were collected at 24 h after infection, and the viral titers (TCID50) were determined on BHK-21 cells three times. The p3D1shRNA, p3D3shRNA, and p3D4shRNA showed a very significant difference compared with the pshRNA and the mock (ANOVA test, ). Error bars indicate standard deviations.
