Degradation Potential of Protocatechuate 3,4-Dioxygenase from Crude Extract of Stenotrophomonas maltophilia Strain KB2 Immobilized in Calcium Alginate Hydrogels and on Glyoxyl Agarose

Table 1

Substrate specificity of immobilized protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. Data shown represent the average of three independent trials ± standard deviation.

Substrate	Relative activity of free enzyme, %	Relative activity of the enzyme immobilized in calcium alginate, %	Relative activity of the enzyme immobilized on glyoxyl agarose, %
Control-protocatechuate	100.0 ± 0.00	100.0 ± 0.00	100.0 ± 0.00
2,3-dihydroxybenzoate	23.60 ± 3.98	149.34 ± 22.94	119.83 ± 7.83
2,4-dihydroxybenzoate	43.50 ± 5.73	96.52 ± 16.78	65.60 ± 13.61
2,5-dihydroxybenzoate	33.46 ± 0.00	158.62 ± 11.71	72.11 ± 12.59
2,6-dihydroxybenzoate	30.51 ± 2.05	90.40 ± 6.09	36.15 ± 7.42
3,5-dihydroxybenzoate	44.33 ± 3.18	113.25 ± 2.81	42.86 ± 13.61
Caffeic acid	34.21 ± 9.65	150.00 ± 16.39	97.08 ± 11.13
3,4-dihydroxyhydrocinnamic acid	29.54 ± 5.42	95.03 ± 7.02	41.11 ± 15.26