Evaluation of the Antioxidant Activity and Antiproliferative Effect of the Jaboticaba (Myrciaria cauliflora) Seed Extracts in Oral Carcinoma Cells
Figure 4
Effects of jaboticaba seed water extract on apoptosis regulatory proteins in HSC-3 cells. HSC-3 cells were treated with 5, 10, 25, 50, and 100 μg/ml of jaboticaba seed extract or ddH2O as a control for 48 h, and then total proteins were isolated. Equal amounts of cell lysates were analyzed for PARP, survivin, Bid, Bcl-xL, and Bcl-2 expression by Western blotting with corresponding antibodies. β-actin served as the loading control. The fold change was calculated as the ratio of the target proteins in the presence of indicated concentration of Jaboticaba seed extract after normalization of the target proteins to β-actin in each lane.
