Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells
Figure 1
Isolation of rat vibrissa dermal papillae and cultivation of DPCs. (a) H&E staining of rat vibrissa hair follicle paraffin sections revealed the anatomic characteristics of dermal papilla. (b) Immunohistochemical staining of frozen sections revealed the expression of ALP in the dermal papilla and outer root sheath. (c) ALP was expressed in the isolated dermal papilla. (d) Spindle-shaped fibrocyte-like cells grew out from the “water-drop” shaped dermal papilla (white arrow). (e) ALP was partly expressed in primary DPCs. (f) Morphology of DPCs at passage 7. Scale bar = 100 μm ((a), (b), (d)), 50 μm ((c), (e)), and 200 μm (f).
