Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells
Figure 6
Isolation and characterization of NCSCs-like cells. (a) After suspension in culture for 7 days, neurosphere-like spheres were generated from DPCs (black arrow), along with non-sphere-forming DPCs (white arrow). ((b)–(e)) Coexpression of Nestin (b) and P75 (c) in spheres were detected by double immunostaining. Nuclei were stained with Hoechst 33342 (d). The images were merged (e). (f) RT-PCR showed a higher expression level of NCSCs-specific markers in sphere-forming DPCs compared with non-sphere-forming DPCs and total DPCs on the same passage. (g) RT-PCR revealed similar expression levels of NTFs in sphere-forming DPCs, non-sphere-forming DPCs, and total DPCs. ((h), (i)) The amount of BDNF (h) and GDNF (i) secreted per 10 thousand sphere-forming DPCs and total DPCs in 5 mL supernatant for 24 h. Scale bar = 20 μm ((a), (b)–(e)). .
