Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue

Table 1

Stability of genes candidates to normalize RT-qPCR for thyroid derived biological material.

Study	Material	Reference gene stability	Method
Santin et al., 2013 [9]	Human normal cells	ACTB>TBP>GAPDH>B2M	NormFinder
Chantawibul et al., 2012 [10]	Human tissues*	GAPDH>ACTB>B2M>RPLIA>HPRT1	NormFinder
Lecchi et al., 2012 [11]	Bovine tissues, including thyroid	SF3A1=HBMS>ACTB	geNorm
		HMBS>ACTB>GAPDH	NormFinder
Li et al., 2011 [12]	Porcine thyroid tissue	18S rRNA>Ubiquitin>Histone H3>ACTB	geNorm
		18S rRNA>Ubiquitin>Histone H3>ACTB>GAPDH	NormFinder
Lisowski et al., 2008 [13]	Bovine thyroid tissue	TBP=HPRT1>YWHAZ>ACTB	geNorm

∗13 goiters, 6 adenomas, 4 carcinomas (2 follicular + 2 papillary), and 2 lymphocytic thyroiditis.