Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178

Table 1

Effect of various compounds on the rPsGrx activity.

Reagent	Concentration	Relative activity (%)
None	—	100.0
DTT	10 mM	41.2
SDS	10 mM	11.3
Thiourea	10 mM	37.8
EDTA	10 mM	79.7
Tween-80	0.2%	53.1
Triton X-100	0.2%	19.0
H2O2	0.2%	9.2
Mg^2+	5 mM	7.0
Zn^2+	5 mM	5.8
Ca^2+	5 mM	17.2
Cu^2+	5 mM	15.0
Ni^2+	5 mM	17.0
Mn^2+	5 mM	39.5
Fe^2+	5 mM	51.5
Fe^3+	5 mM	51.3
K^+	5 mM	26.8
Na^+	0.5 M	69.1
Na^+	1.0 M	37.0
Na^+	1.5 M	11.2