The Role of Neurotrophic Factors Conjugated to Iron Oxide Nanoparticles in Peripheral Nerve Regeneration: In Vitro Studies
Figure 6
Myelinated organotypic DRG cultures exposed to conjugated-GDNF (left column) versus nonmyelinated cultures (right column). (a) and (b): Representative immunofluorescence images of a myelinated (day 12) versus nonmyelinated cultures, respectively. Neurites appear in red (antineurofilament staining) and myelin sheaths appear in green (antimyelin basic protein staining). (c) and (d): Phase-contrast microscope images of a heavily myelinated (day 16) versus nonmyelinated culture, respectively. Note the arrows in (c) which point to a single birefringent myelinated nerve fibre (appears as a bright fibre confined by two dark lines along both its sides due to the optical property of the myelin sheaths lipids). The arrows in (d) point to nonmyelinated fibres. (e): Staining of myelinated nerve fibres with Luxol Fast Blue. (f): Phase-contrast microscope image (through a green filter) of individual DRG cells in a nonmyelinated culture.
