Recombinant Human Factor IX Produced from Transgenic Porcine Milk
Figure 1
Electrophoretic analysis of rhFIX from transgenic porcine milk. (a) Nonreducing SDS-PAGE analysis. All lanes contain 10 μg of protein. Lane M: molecular mass markers, Lane 1: whey. Lane 2: Q-Sepharose. Lane 3: heparin-Sepharose. Lane 4: Con A-Sepharose. (b) Isoelectric focusing (IEF) analysis. IEF was performed using Invitrogen pH 3–7 gels. Proteins were detected with Coomassie blue stain. Lane M: pI markers, Lane 1: plasma-derived FIX (pdFIX), Lane 2: FIX recombinantly produced from CHO cells (CHO-FIX), Lane 3: rhFIX purified from transgenic porcine milk (tgFIX).
