Recombinant Human Factor IX Produced from Transgenic Porcine Milk

Figure 3

HPLC analysis of tgFIX digested by trypsin and PNGase F. Tn denotes the 𝑛th enzymatic peptide assigned from the 𝑁-terminal of tgFIX. For example, T1 indicates the first theoretical tryptic peptide from the protein 𝑁-terminal. Tn-Tn+1 or Tn-Tn+2 indicate peptides resulting from one or two missed cleavages, respectively. The collection time and matched peptide sequence are summarized in Table 4. Each peptide sequence was supported by MS/MS data and molecular weight match. A Mascot probability-based scoring algorithm was used to evaluate the identification.