Research Article
Recombinant Human Factor IX Produced from Transgenic Porcine Milk
Table 1
Summary of a representative purification of tg-FIX from transgenic porcine milk.
| | Step | Volume (mL) | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification (fold) | Yield (%) | FIXa/FIX (%) |
| | Whey | 409 | 11342 | 34026 | 3 | 1 | 100 | 19 | | Q-Sepharose | 100 | 211 | 25531 | 121 | 40 | 75 | 18 | | Heparin-Sepharose | 23 | 42 | 10752 | 256 | 85 | 32 | 11 | | Concanavalin A-Sepharose | 20 | 12 | 3516 | 293 | 98 | 10 | 1 |
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