Recombinant Human Factor IX Produced from Transgenic Porcine Milk

Table 4

Peptide matching of tgFIX digested by trypsin plus PNGase F. Fractions were collected manually from HPLC separation. This represents data from the same experiment as shown in Figure 3. The peptide identities suggested by LC-MS/MS analysis are listed. Those fractions in which no peptide was identified are removed from the list.

Fraction number	Time	Peptide number	Position	Observed 𝑚/𝑧	Match peptides
1	35.1~35.8	T16	135–142	418.1808	VSVSQTSK
5	38.8~40.2	T11	95–100	871.442	CEQFCK
6	42.4~43.1	T13	107–116	615.7483	VVCSCTEGYR
7	48.0~48.8	T12-T13	101–116	620.5777	NSADNKVVCSCTEGYR
8	50.2~51.2	T38	395–400	744.471	YGIYTK
		T37-T38	393–400	465.2822	GKYGIYTK
10	54.0~54.8	T25	253–265	752.9462	IIPHHNYNAAINK
11	57.0~57.8	T9-T10	81–94	565.2617	NCELDVTCNIKNGR + Deamidated (NQ)
12	59.4~60.2	T22-T23	229–248	580.8007	ITVVAGEHNIEETEHTEQKR
13	60.3~61.2	T9	81–91	683.3281	NCELDVTCNIK
14	62.8~63.8	T20	202–214	698.3452	VDAFCGGSIVNEK
15	63.9~64.6	T6	38–43	406.2174	TTEFWK
16	64.8~65.8	T40-T41	404–411	540.3281	YVNWIKEK
		T15	123–134	688.851	SCEPAVPFPCGR
17	67.4~68.0	T28	302–312	586.8006	FGSGYVSGWGR
18	68.2~68.8	T40	404–409	411.7751	YVNWIK
19	70.4~71.4	T21	215–228	785.9102	WIVTAAHCVETGVK
20	71.6~72.6	T40–T43	404–415	761.9668	YVNWIKEKTKLT
21	80.8~81.4	T35	342–358	679.6353	FTIYNNMFCAGFHEGGR + Oxidation (M)
22	81.4~82.8	T30-T31	317–327	638.3962	GRSALVLQYLR
		T35	342–358	679.6486	FTIYNNMFCAGFHEGGR + Oxidation (M)
24	82.8~83.4	T35	342–358	679.6478	FTIYNNMFCAGFHEGGR + Oxidation (M)
25	83.4~84.0	T31	319–327	531.8453	SALVLQYLR
26	84.1~85.0	T31	319–327	531.8246	SALVLQYLR
		T35	342–358	674.6462	FTIYNNMFCAGFHEGGR + Deamidated (NQ)
		T34-T35	339–358	779.7193	STKFTIYNNMFCAGFHEGGR
27	85.4~86.2	T35	342–358	1010.9647	FTIYNNMFCAGFHEGGR
		T35	342–358	679.6509	FTIYNNMFCAGFHEGGR + Oxidation (M)
		T8	64–80	1062.4471	DDINSYECWCPFGFEGK
		T34-T35	339–358	589.0386	STKFTIYNNMFCAGFHEGGR + Oxidation (M)
28	86.4~87.6	T35	342–358	1010.9542	FTIYNNMFCAGFHEGGR
		T35	342–358	679.6524	FTIYNNMFCAGFHEGGR + Oxidation (M)
29	88.6~89.6	T19	181–201	742.7371	VVGGEDAKPGQFPWQVVLNGK + Deamidated (NQ)
30	90.2~91.0	T19	181–201	742.3987	VVGGEDAKPGQFPWQVVLNGK
31	92.2~93.2	T19	181–201	742.7443	VVGGEDAKPGQFPWQVVLNGK + Deamidated (NQ)
34	94.2~95.2	T19-T20	181–214	901.2115	VVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEK
37	106.0~107.0	T31–T33	319–338	781.7831	SALVLQYLRVPLVDRATCLR
		T26	266–293	1076.8988	YNHDIALLELDEPLVLNSYVTPICIADK
38	107.4~108.2	T36-T37	359–394	957.4534	DSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGK
39	108.4~109.2	T36	359–392	1214.5544	DSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMK
43	115.6~116.6	T18	146–180	1313.9320	AEAVFPDVDYVNSTEAETILDNITQSTQSFNDFTR + 2 Deamidated (NQ)