Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment

Figure 5

Agarose gel electrophoresis of PCR amplification products for twenty-two putative transformed and control samples from Agrobacterium and particle bombardment transformation methods. Lanes denoted as M represent the 1 kb DNA ladder (Fermentas); N represent the amplification of negative control (untransformed embryoids); section P represents the amplification of positive controls for gus and bar genes. Lanes 1–3 and 4–15 are amplification products for gus genes from Agrobacterium-mediated and particle bombardment methods, respectively. Meanwhile, lanes 16–18 and 19–22 are amplification products for bar genes from Agrobacterium-mediated and particle bombardment methods, respectively.