Yokukansan, a Traditional Japanese Medicine, Adjusts Glutamate Signaling in Cultured Keratinocytes
Figure 5
YKS regulates NMDAR2D and GLAST mRNA expression in cultured keratinocytes. Human keratinocytes were incubated with 125–500 μg/mL of YKS or medium alone until the cells became confluent. Following incubation, total RNA was extracted and reverse transcribed into cDNA and then RT-PCR was performed. The NMDAR2D (a) and GLAST (b) mRNA expression levels were measured using RT-PCR. NMDAR2D mRNA expression decreased in response to YKS stimulation (125–500 μg/mL) in a concentration-dependent manner. GLAST mRNA levels increased following the addition of YKS in a concentration-dependent manner. The measurements were compared between the stimulated and nonstimulated cells (Cont; control). () , , and .
