Host-Range Dynamics of Cochliobolus lunatus: From a Biocontrol Agent to a Severe Environmental Threat
Figure 2
(a) Archetypal brown-to-black leaf spot disease caused by Cochliobolus lunatus on potato cv. Kufri Jyoti as previously validated by Koch’s postulates [20]. (b) Agarose gel electrophoresis for PCR products (475 bp) from rDNA locus of Cochliobolus lunatus strains separated on a 2.5% agarose gel. Lane-1 DNA ladder and lane-2, -3, -4, -5, and -6 are Cochliobolus lunatus strains with GenBank accessions numbers JX512810, JX512809, JX907827, JX477595, and JX907828, respectively. (c) Molecular phylogenetic analysis by maximum likelihood method based on the K2 + G substitution model [21]; AIC is 871.49; BIC is 1093.89; the highest log likelihood is −429.87 and bootstrap values ≥50% from 1000 iterations are shown. Subcluster I contain strains of Cochliobolus lunatus which causes foliar necrosis of potato.
