Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells
Figure 10
miR-146a mimic enhanced the growth inhibitory effect of EGFR targeting agents in HCC HepG2 cells. HepG2 cells (2.5 × 103 cells per well in 96-well plate) were cultured for 24 h then transfected with miR-146a mimic and negative control (200 nM). Meanwhile, gefitinib, erlotinib, and cetuximab were added and the cells were cultured up to another 96 h. MTS was performed as above and the proliferation inhibition rate was calculated. Bliss independence curve indicated the theoretical situation in which the combinational effect of miR-146a mimic and other agents was exactly additive. , compared to agent alone. Bliss independence criterion was applied to analyze the theoretical additive effect.
