Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR

Table 4

Analytical sensitivity of the three automated nucleic acid extraction systems.

Virus strain; dilution factor

ADV-3 (detected/replicates)

FluA (detected/replicates)

RSV-A (detected/replicates)

10^−6

10^−7

10^−8

10^−9

10^−10

10^−5

10^−6

10^−7

10^−8

10^−9

10^−4

10^−5

10^−6

10^−7

10^−8

QIAcube (QIAGEN)

5/5

3/5

0/5

0/5

0/5

5/5

2/5

1/5

0/5

0/5

5/5

4/5

0/5

0/5

0/5

EZ1 (QIAGEN)

5/5

5/5

0/5

0/5

0/5

5/5

5/5

2/5

1/5

0/5

5/5

5/5

3/5

1/5

0/5

NIMBUS (Seegene)

5/5

4/5

0/5

0/5

0/5

5/5

4/5

0/5

0/5

0/5

5/5

2/5

1/5

0/5

0/5