Single Amino Acid Arginine Deprivation Triggers Prosurvival Autophagic Response in Ovarian Carcinoma SKOV3

Figure 5

Effect of arginine deprivation and CQ treatment on accumulation of autophagosomal protein LC3II and phosphorylation of mTOR substrates. After indicated periods of treatment, the cells were washed and harvested for WB analysis. 50 μg of total sample protein was loaded per lane. 𝛽-Actin served as the loading standard.