Research Article
Analgesic and Anti-Inflammatory Activities of Leaf Extract of Mallotus repandus (Willd.) Muell. Arg.
Table 1
Phytochemical constituents identified in methanol extract of M. repandus leaf (MLM).
| Phytochemical | Name of the tests | Observed changes | Result |
| Alkaloids | Mayer’s test | Creamy white precipitate | − | Hager’s test | Yellow crystalline precipitate | + | Wagner’s test | Brown or deep brown precipitate | − | Dragendorff’s test | Orange or orange-red precipitate | − | Tannic acid test | Buff color precipitate | − | Carbohydrates | Molisch’s test | A red or reddish violet ring is formed at the junction of two layers and on shaking a dark purple solution is formed | + | Barfoed’s test (general test for monosaccharides) | Red precipitate | − | Fehling’s test | A red or brick-red precipitate | − | Benedict’s test | | + | Test for combined reducing sugar | A brick-red precipitate | − | Glycosides | General test | Yellow color | − | Bromine water test | yellow precipitate | + | Test for glucoside | Production of brick-red precipitation (carried out with the hydrolyzed extract) | − | Flavonoids | Alkaline reagent test | Red color |
+/− | Shinoda test (magnesium hydrochloride reduction test) | Green to blue color | + | Zinc hydrochloride reduction test | Red color after few minutes | − | Saponins | Frothing test | Formation of stable foam | + | Steroids | Libermann-Burchard’s test | Greenish color | − | Tannins | Lead acetate test | A yellow or red precipitate | + | Ferric chloride test | Blue green color | − | Alkaline reagent test | Yellow to red precipitate | + | Terpenoids | Salkowski test | Yellow color appears at the lower layer | − |
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(+) = presence; (−) = absence; (+/−) = presence or absence not ascertained.
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