Disruption of HPV16-E7 by CRISPR/Cas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells

Figure 3

Disruption of the E7 gene could significantly enhance apoptosis monitored by FCM in SiHa and Caski cells, but not in C33A and HEK293 cells. gRNA-1/Cas9 ((a), (c)), gRNA-2/Cas9 ((b), (d)), gRNA-3/Cas9 ((e), (g)), and gRNA-4/Cas9 ((f), (h)) induced apoptosis rate in SiHa, Caski, C33A, and HEK293 cell lines ( compared to blank control, per Student’s -test).

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