Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma
Figure 6
Effect of the mitochondrial complex III inhibitor on formation in human PRP. The ESR spectra show (a) the effect of a solvent control, (A) 0.6% DMSO, and 10, 30 (data not shown), and (B) 100 M antimycin in the presence of 100 mM DMPO for 30 min on formation in human PRP (approximately platelets/mL). The ESR spectra are labeled to show their components: DMPO- adduct (*). The ESR signal intensity rate and data shown in the bar chart (b) are expressed as the means ± SEM (). compared with the solvent control. The instrument parameters were identical to those shown in Figure 1.
