Sequence Variation in the E2-Binding Domain of HPV16 and Biological Function Evaluation in Tunisian Cervical Cancers
Figure 2
(a) RT-PCR products of HPV16 E6/E7 in episomal forms from cervical positive samples with and without T310K variation. Lanes 1–6 are shown as examples (amplicon length 300 bp). M: size markers of 50 bp ladder; N: negative control (no template); C: positive control (HPV16 plasmid). (b) Specific gene expression and quantification of HPV16 E6/E7 relative to -actin expression levels in episomal forms with and without T310K variation. Bars represent mean ± SEM. Significantly elevated expression (), Student’s -test.
