Application of PCR-ELISA in Molecular Diagnosis

Table 1

Comparisons between 3 different detection methods; conventional PCR with agarose gel electrophoresis, PCR-ELISA and qPCR.

Comparison	Conventional PCR	PCR-ELISA	qPCR
Equipment required	Standard laboratory equipment	Standard laboratory equipment	Requires fluorescence detection instrument
Reagent costs	Low	Moderate	Costly
Detection limit	1–10 ng/μL	0.01 ng/μL	0.25 pg/μL
Quantitative ability	Not quantitative	Semi-quantitative	Quantitative