Glycoprotein IIb/IIIa and P2Y₁₂ Induction by Oligochitosan Accelerates Platelet Aggregation

Figure 3

Expression of P2Y₁₂ upon the adherence of chitosan biomaterials in vitro. Protein was extracted from PRP exposed to chitosan, and the expression of P2Y₁₂ was determined by western blotting (Figure 3(a)). The protein bands were visualized using an image analyzer and band density was analyzed using ImageJ software, with the fold-change differences in expression compared with blood alone written below each band. Figure 3(b). The expression levels of P2Y₁₂ upon the presence of chitosan biomaterials are shown as the fold change in comparison with blood alone (1-fold). The blots shown are representative of the mean ± SEM from three independent experiments (). The dotted line depicts the expression level in the blood alone (1-fold).

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