Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors
Figure 2
K1-5 and cell viability. 104 hepatoma cells were seeded on 96-well plates, transfected with Lipofectamine and pCMVTNT-vectors or treated with plain medium and incubated for twenty four hours; = 4, < 0.05 (plain medium/pK1-5), < 0.05 (pMock/pK1-5). Optical density of medium treated cells was set as 100%. Data are shown as mean percentage ± SEM. (a) Supernatants were removed, and MTT was added and incubated. Supernatants were removed, MTT was solubilized with DMSO, and OD was measured at 560 nm. (b) Cells were labeled with BrdU, incubated for 24 hours, and fixed with a specific antibody and optical density was measured at 560 nm. (c) Cytoplasmatic histone-associated DNA fragments were quantified with the Cell Death Detection kit (Roche diagnostics) and optical density measured at 405 nm.
