Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors
Figure 8
In vivo effects of pK1-5 on TNF-alpha expression. Mice with subcutaneous tumours were treated with NaCl ( = 5), DOTAP ( = 6), pMock ( = 6), or pK1-5 ( = 5) and sacrificed after ten days. Tumours and livers were explanted and homogenized and protein concentrations were evaluated. TNF-alpha levels were quantified with a murine TNF-alpha ELISA. Data are presented as mean pg TNF-alpha/100 µg protein. < 0.05 (compared to DOTAP), < 0.05 (compared to pMock). (a) Tumour samples, (b) liver samples, and (c) tumour sections were stained against TNF-alpha and counterstained with hematoxyline. Exemplary photomicrographs of animals treated with NaCl, DOTAP, pMock, and pK1-5. TNF-alpha is shown by brown staining.
