Plastic Changes in the Spinal Cord in Motor Neuron Disease
Figure 4
H&E staining of lamina X. (a) Atlas plate [25] of the lumbar cord (L4) used as the reference for representative pictures. (b) Representative high magnification of H&E stained pictures showing L4 lamina X, treated with either saline or lithium. The cell number counted within lamina X is equivalent for WT mice treated with either saline (WT Sal) or lithium (WT Li). On the other hand, the cell number is increased in the lamina X of G93A mice treated with saline (G93A Sal) or lithium (G93A Li). When counting the cell number it is evident that, as reported in the histogram (c), the cell number in lamina X is mostly increased in G93A mice receiving lithium. The increase in the cell number occurring within lamina X of G93A mice is better visualized at the level of the ependymal layer. Dashed lines draw the border of the lamina X. * compared with other groups. Scale bar = 30 μm.
