Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation
Figure 4
ENERGI-F704 decreases LPS-induced NO, iNOS, COX-2, and PGE2 production in BV2 cells. BV2 cells were stimulated with 200 ng/mL LPS and subsequently treated with either ENERGI-F704 or AICAR for 24 h. (a) Levels of NO in the culture of BV2 cell were determined. (b) After treatments, cell lysates were used to determine the level of iNOS and COX-2 using Western blotting. (c) LPS-treated BV2 cells were treated with ENERGI-F704 in cotreatment with or without compound C for 24 h. Cell lysates were used to determine the level of iNOS and COX-2 using Western blotting. (d) LPS-induced BV2 cells were treated with ENERGI-F704 in cotreatment with or without compound C for 48 h, and the level of PGE2 in the culture medium was determined. Data are presented as the mean ± SEM of three independent experiments (two-way ANOVA; , , and , , , and ; specific comparison to LPS-treated control). Representative images of three independent experiments are shown.
