Differential Expression and Immunolocalization of Antioxidant Enzymes in Entamoeba histolytica Isolates during Metronidazole Stress

Figure 7

Immunolocalization of peroxiredoxin in clinical isolates of E. histolytica given short term metronidazole stress. The images from left to right in each panel show phase contrast, peroxiredoxin stained by Alexa 488, Hoechst 3342 for nuclear staining, and the merged image. In normal conditions Peroxiredoxin localized to the membrane and to the nucleus in axenic clinical isolate MS96 (AX) (panel (1)). After 24 and 48 hours of metronidazole stress peroxiredoxin localized to the membrane with loss of nuclear staining (panels (2) and (3)). Xenic isolates MS96 (X) and 654 showed loss of nuclear staining with metronidazole shock (panels (4) to (7)). Panels (6) and (7) show cytoplasmic localization of CaBp1 during the stress.