Amarogentin, a Secoiridoid Glycoside, Abrogates Platelet Activation through PLCγ2-PKC and MAPK Pathways
Figure 2
Effects of amarogentin on collagen-induced PLCγ2 and PKC activation. Washed platelets (1.2 × 109 cells/mL) were incubated with solvent control (0.5% DMSO) or amarogentin (15 μM~120 μM) for 3 min. Then, 1 μg/mL collagen was added to induce the phosphorylation of PLCγ2 (a) and p47 (b) for 5 min and 10 min, respectively. ((a) and (b)) The subcellular extracts were analyzed for PLCγ2 (a) and p47 (b) by western blotting. (c) Washed platelets (3.6 × 108 cells/mL) were incubated with solvent control (0.5% DMSO) or amarogentin (15 μM~120 μM) for 3 min and then treated with 150 nM PDBu to induce platelet aggregation. Data ((a) and (b)) are presented as the mean ± SEM (). and , compared with the solvent control group (resting); , compared with the positive control group (collagen only). Profiles (c) are representative of 3 independent experiments.
