Rapid Degradation of Hfq-Free RyhB in Yersinia pestis by PNPase Independent of Putative Ribonucleolytic Complexes
Figure 1
Effects of RNases and an ancillary RNA-modifying enzyme on the transcriptional level of Y. pestis RyhB1 and RyhB2 in the background. RyhB1 and RyhB2 were detected by Northern blotting using 5 μg of total RNA extracted from Y. pestis grown to exponential phase in BHI medium upon treatment with 100 μM DIP treatment for 20 min. 5S rRNA was used as a negative control. Lanes 1–8 represent WT (lane 1), hfq mutant (lane 2), double mutants lacking hfq, and another gene encoding either endoribonucleases (RNase E910, RNase G) (lanes 4 and 5), exoribonucleases (RNase III, PNPase, and RNase II) (lanes 3, 6, and 8), or ancillary RNA-modifying enzyme (polyA polymerase) (lane 7).
