Barcoding Melting Curve Analysis for Rapid, Sensitive, and Discriminating Authentication of Saffron (Crocus sativus L.) from Its Adulterants

Table 2

The primers sequence and PCR reaction in this paper.

Markers	Primer	Sequences 5′ to 3′	Real-time PCR reaction conditions
trnH-psbA	psbAF	GTTATGCATGAACGTAATGCTC	50°C 2 min; 95°C 30 sec; 95°C 5 sec, 58°C 34 sec, 40 cycles
	trnHR	CGCGCATGGTGGATTCACAATCC
rbcL	1F	ATGTCACCACAAACAGAAAC	50°C 2 min; 95°C 30 sec; 95°C 5 sec, 60°C 34 sec, 40 cycles
	724R	TCGCATGTACCTGCAGTAGC
ITS2	ITS2	ATGCGATACTTGGTGTGAAT	50°C 2 min; 95°C 30 sec; 95°C 5 sec, 60°C 34 sec, 40 cycles
	ITS3	GACGCTTCTCCAGACTACAAT
matK	3F	CGTACAGTACTTTTGTGTTTACGAG	50°C 2 min; 95°C 30 sec; 95°C 5 sec, 50°C 10 sec, 54°C 34 sec, 40 cycles
	1R	ACCCAGTCCATCTGGAAATCTTGGTTC
trnL-F	trnL	GGAAATCGGTAGACGCTACG	50°C 2 min; 95°C 30 sec; 95°C 5 sec, 60°C 34 sec, 40 cycles
	trnF	ATTTGAACTGGTGACACGAG