Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective

Figure 4

Growth curves of Gram-positive strains, (a) Bacillus subtilis and (b) Staphylococcus aureus, and Gram-negative bacteria, (c) Enterobacter aerogenes and (d) Salmonella choleraesuis, grown in nutrient broth containing the Cry8Ka5, Cry1Ac, and ovalbumin, all at a concentration of 1,000 𝜇g/mL, and non-protein controls, sodium 50 mM phosphate buffer, pH 7.0, and 0.4% formol. The coefficients of variation for each point were ≤10%. For all readings Abs₆₀₀, Cry8Ka5, and Cry1Ac showed no significant difference (𝑃>0.05, one-way ANOVA) compared to the controls, ovalbumin, or 50 mM sodium phosphate buffer, pH 7.0.

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