Immobilized Lentivirus Vector on Chondroitin Sulfate-Hyaluronate Acid-Silk Fibroin Hybrid Scaffold for Tissue-Engineered Ligament-Bone Junction
Figure 4
(a, b) The viability of MSCs seeded on scaffold evaluated by confocal microscope at 1-week (a) and 2-week (b) culture period (FDA/PI staining; green represents live cells; red represents dead cells). (c, d) The transfection of MSCs observed by fluorescence microscope at 1-week (c) and 2-week (d) culture period (×40). (e) Nonadherent cells count at 0, 0.5, 1, 2, 4, and 8 h time points after cell seeding (mean ± SD, , ). (f) The value of alamar blue assay on days 1, 3, 5, 7, 9, 11, 13, and 15 after cell seeding (, ).
