Hepatitis B Virus X Upregulates HuR Protein Level to Stabilize HER2 Expression in Hepatocellular Carcinoma Cells
Figure 4
The increased HER2 protein expression was responsible for the migration ability of HBx-expressing HCC cells. ((a), (b), (c), and (e)) Hep3Bx cells were transiently transfected with either si-control or si-HER2 for 4 days. Then, cells were either harvested or reseeded for further experiments. Gene silence of HER2 expression was confirmed by Western blot (a) (). The relative growth rate was determined by crystal violet staining (b) (). The migration of Hep3Bx cells was examined by Transwell migration assay for 48 hrs. The representative pictures of migrated cells were visualized and quantified (c) (). The expressions of metastatic factors were examined by Western blot (e) (). (d) The expressions of metastatic factors in both Hep3B and Hep3Bx cells were examined by Western blot ().
