In Vitro Antiplasmodial Activities and Synergistic Combinations of Differential Solvent Extracts of the Polyherbal Product, Nefang

Table 5

In vitro antiplasmodial activity (EC₅₀) of paired extracts exhibiting additive interaction (0.7 < CI < 0.7) at equipotency ratios.

Number	Extract combination	EC50 ratio (µg/mL)	EC50 ratio in combination (µg/mL)	FIC50 A	FIC50 B	Fold increase	Combination index (CI)
1	MiB/MiL-EtOH	14/16.34	6.88/8.02	0.49	0.49	2.03/2.03	0.98
2	MiB/Cp-EtOH	14/121.60	7.99/69.36	0.57	0.57	1.75/1.75	1.14
3	MiB/Og-EtOH	14/121.50	5.20/45.32	0.37	0.37	2.69/2.68	0.74
4	MiL/Pg-EtOH	16.34/23	7.94/11.18	0.48	0.48	2.06/2.06	0.96
5	MiL/Cc-EtOH	16.34/54.84	11.01/36.94	0.67	0.67	1.48/1.48	1.34
6	MiL/Cs-EtOH	16.34/86.08	9.22/48.55	0.56	0.56	1.77/1.77	1.12
7	MiL/Og-EtOH	16.34/121.50	5.89/43.95	0.36	0.36	2.77/2.76	0.72
8	Pg/Cp-EtOH	23/121.60	10.85/57.38	0.47	0.47	2.12/2.12	0.94
9	Pg/Cs-EtOH	23/86.08	13.77/51.52	0.59	0.59	1.67/1.67	1.19
10	Pg/Og-EtOH	23/121.50	11.31/59.97	0.49	0.49	2.03/2.03	0.98
11	Cc/Cs-EtOH	54.84/86.08	35.03/54.99	0.64	0.64	1.57/1.57	1.28
12	MiB-EtOH/MiB-Aq	14/34.58	7.75/19.13	0.55	0.55	1.81/1.81	1.10
13	MiB/MiL-Aq	34.58/40.04	19.68/22.78	0.57	0.57	1.76/1.76	1.14
14	MiB/Cc-Aq	34.58/141	16.87/68.79	0.49	0.49	2.05/2.05	0.98
15	MiB/Og-Aq	34.58/118.90	14.91/51.28	0.43	0.43	2.32/2.32	0.86
16	MiL/Cc-Aq	40.04/141	14.31/50.39	0.36	0.36	2.80/2.80	0.72
17	MiL/Og-Aq	40.04/118.90	16.95/50.32	0.42	0.42	2.36/2.36	0.84

Results presented as mean, 𝑛=3.
EtOH: ethanol; Aq: aqueous.