Adrenomedullin Deficiency and Aging Exacerbate Ischemic White Matter Injury after Prolonged Cerebral Hypoperfusion in Mice

Figure 3

Serial changes in OLGs and OPCs after prolonged cerebral hypoperfusion in young groups. (a) Photomicrographs of GSTπ-, PDGFRα-, Iba-1-, and iNOS-positive cells in the corpus callosum before and after BCAS. Scale bars = 20 μm (GSTπ, Iba-1), scale bars = 50 μm (iNOS), and scale bars = 100 μm (PDGFRα). ((b)–(e)) Results of quantitative analysis of the number of GSTπ- (b), PDGFRα- (c), Iba-1- (d), and the density of iNOS- (e) positive cells in the corpus callosum before and at days 3, 7, 14, and 28 after BCAS. Data are mean ± SEM of five mice in each group. ^∗𝑃<0.05, ^∗∗𝑃<0.001, compared with the preoperation group (pre). ^†𝑃<0.05, ^††𝑃<0.001, compared with the same time points. Pre: preoperation.