Integrative Analysis of miRNA-mRNA and miRNA-miRNA Interactions
Figure 2
(a) miRNA expression analysis and (b) and further qRT-PCR validation. (a) The fold change values (log 2) differ in the variety of miRNA sequences involved. “The-most” indicates the most abundant and dominant isomiR sequence. “All-isomiRs” indicates sum of all isomiRs. “The-canonical” indicates the reference miRNA sequence in the miRBase database. The canonical miRNA sequence may be consistent or inconsistent with the most abundant isomiR sequence. Different methods of estimation may produce different fold change values (log 2), but they always show consistent deregulation patterns. (b) Further RT-PCR validation is performed for miR-15b, miR-103a, and miR-106b, and the experimental results show consistent deregulation patterns. “*” indicates that the value is less than 0.05.
