Enhanced Efficacy of Bleomycin in Bladder Cancer Cells by Photochemical Internalization
Figure 1
Cellular uptake of is dose-dependent, its cytotoxicity is dependent upon dose of blue light, and bleomycin cytotoxicity is cell type-dependent. (a) Cellular accumulation at increasing concentrations of in A431, T24, and AY-27 cells. After 18 h the fluorescence was normalized to fluorescence intensity per cell by DRAQ5-staining. The data are from one representative experiment out of two (mean of 12 wells ± SEM). (b) Cytotoxicity of increasing blue light doses (435 nm) in A431, T24, and AY-27 cells exposed to 0.3 μg mL−1 for 18 h. The data are from one representative experiment out of three (mean of 24 wells ± SEM). Light doses selected for further PCI experiments are indicated with arrows. (c) Dose response to bleomycin determined as cell growth after 48 h. A431, T24, and AY-27 cells were treated with different bleomycin doses for 4 h. The data are from one representative experiment out of four (mean from 48 wells ± SEM). Tables of values show statistical significance using two-tailed Student’s -test.
