FC-98 Regulates TLR9-Mediated of CXCL-10 Expression in Dendritic Cells via MAPK and STAT1 Signaling Pathway
Figure 2
The effect of FC-98 on the cell viability and apoptosis of BMDCs. (a) BMDCs were treated with FC-98 in the range of 0–400 M for 24 h and then the CCK-8 assay was applied to detect the cell viability. (b) Cells were stained with Annexin V and PI followed by flow cytometry analyses. Cells stained with FITC-Annexin V alone (Annexin V+/PI−) were considered in early apoptosis, whereas those stained with both FITC-Annexin V and PI (Annexin V+/PI+) were considered in the advanced stages of apoptosis or necrosis. The FITC-Annexin V and PI double-negative (Annexin V−/PI−) cells were considered alive. Data are expressed as mean ± SD of three independent experiments. ** versus control (Con) group.
