FC-98 Regulates TLR9-Mediated of CXCL-10 Expression in Dendritic Cells via MAPK and STAT1 Signaling Pathway

Figure 3

FC-98 inhibited CpG-induced upregulation of surface markers without influence on endocytosis. (a) BMDCs were treated with indicated concentrations of FC-98 for 2 h, and then CpG (1 𝜇M) were added in. After 24 h, the expressions of CD40, CD80, CD86, and MHCII were analyzed by flow cytometry. (b) BMDCs were treated with or without FC-98 and then 0.5 mg/mL FITC-Dextran was added for another 1 h at 37°C (4°C as control) and analyzed by flow cytometry. Data are shown as mean ± SD of three independent experiments. ^#𝑃<0.05, ^###𝑃<0.001 versus control group; *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 versus CpG only group.

(a)

(b)