FC-98 Regulates TLR9-Mediated of CXCL-10 Expression in Dendritic Cells via MAPK and STAT1 Signaling Pathway
Figure 5
FC-98 inhibited activation of MAPK and STAT1 to downregulate the CXCL-10 expression. (a) Left: BMDCs were pretreated with FC-98 for 2 h, followed by 30 min CpG treatment; the phosphorylation of of MAPK (ERK, JNK, and p38) signaling pathway was analyzed by western blot. The results shown are representative experiments from three independent assays. Right: BMDCs were cotransfected with 100 ng pAP-1 luciferase reporter plasmid and 10 ng pRL-TK-Renilla luciferase. Total amounts of plasmid DNA were equalized using empty control vector. After 24 h of culture, cells were pretreated with FC-98 for 1 h and then stimulated with 1 M CpG for another 6 h. Luciferase activity was measured and normalized by Renilla luciferase activity. Data are shown as mean ± SD of three independent assays. ### versus control group; *, *** versus CpG-only group. (b) Left: experiments were duplicated as described in (a), left, and phosphorylated STAT1 (p-STAT1) and total STAT1 were detected by western blot. The results shown are representative experiments from three independent assays. Right: the results of western blot were analysed by Image J, and the relative intensity was showed. ### versus control group; ** versus 2 (CpG-only group).
