Hyperuricemic PRP in Tendon Cells

Table 1

Real-time PCR primers used in this study.

Gene	Forward primer (5′→3′)	Reverse primer (5′→3′)	(°C)
SCX	CAGCCCAAACAGATCTGCACCTT	CTGTCTTTCTGTCGCGGTCCTT	58
DCN	GGTGGGCTGGCAGAGCATAAGT	TGTCCAGGTGGGCAGAAGTCA	58
TNMD	GAAGCGGAAATGGCACTGATGA	TGAAGACCCACGAAGTAGATGCCA	60
COMP	CCGACAGCAACGTGGTCTT	CAGGTTGGCCCAGATGATG	55
ACAN	ACAGCTGGGGACATTAGTGG	GTGGAATGCAGAGGTGGTTT	55
SOX9	AGCGAACGCACATCAAGAC	GCTGTAGTGTGGGAGGTTGAA	55
COL1A1	GGCAACAGCCGCTTCACCTAC	GCGGGAGGACTTGGTGGTTTT	58
COL3A1	CACGGAAACACTGGTGGACAGATT	ATGCCAGCTGCACATCAAGGAC	58
COL2A1	AACCAGATTGAGAGCATCCG	AACGTTTGCTGGATTGGGGT	55
HAS2	GTCCCG GTGAGACAGATGAG	ATGAGGCTGGGTCAAGCATAG	58
IL-1b	TCCAAGGGGACAGGATATGGAGCA	AGGCCCAAGGCCACAGGTATTT	58
IL-6	GAGGCACTGGCAGAAAACAACC	CCTCAAACTCCAAAAGACCAGTGATG	58
IL-8	CTGTCTGGACCCCAAGGAAAACT	GCAACCCTACAACAGACCCACAC	57
COX1	GGTTTGGCATGAAACCCTACACCT	CCTCCAACTCTGCTGCCATCT	58
COX2	AACTGCGCCTTTTCAAGGATGG	TGCTCAGGGACTTGAGGAGGGT	58
TGF-beta1	GAGGTCACCCGCGTGCTAATG	CACGGGTTCAGGTACCGCTTCT	58
GAPDH	GCATTGCCCTCAACGACCACT	CCATGAGGTCCACCACCCTGT	58
TBP	TGCACAGGAGCCAAGAGTGAA	CACATCACAGCTCCCCACCA	58

Scleraxis (SCX), decorin (DCN), tenomodulin (TNMD), cartilage oligomeric protein (COMP), aggrecan (ACAN), SRY (sex determining region Y)-box 9 (SOX9), collagen type I alpha 1 (COL1A1), collagen type 3 alpha 1 (COL3A1), collagen type II alpha 1 (COL2A1), hyaluronan synthase 2 (HAS2), interleukin 1, beta (IL-1b), interleukin-6 (IL-6), interleukin-8 (IL-8), cytochrome c oxidase 1 (COX1), cytochrome c oxidase 2 (COX2), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and TATA box binding protein (TBP).