Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides
Figure 5
(a) Enzyme preparation protein versus rate of reaction product formation. (b) Time of incubation versus amount of product formed. A time course experiment was performed at various intervals of time, namely, 0 hours, 15 minutes, 30 minutes, 1 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, 12 hours, and 24 hours with 37°C as the temperature of incubation. (c) Assay pH versus catalytic activity of GsSGT. (d) substrate saturation curve of GsSGT for aglycon (ergosterol). (e) Thermostability of the recombinant GsSGT. (f) Optimum temperature for enzymatic actvity of GsSGT.
